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    Abstract

  • A study was carried out at the Biotechnology-cum-Tissue Culture Centre, OUAT, Bhubaneswar, India, to standardise the surface sterilization time and media supplements for culture establishment and better proliferation and media supplements for root formation of a wonderful landscape shrub B.galpinii which is difficult to propagate. For in vitro studies, single node explants (6-8mm size) were taken from branches of Bauhinia galpinii plants. The explants were surface sterilized with 0.1% HgCl2 for different time periods i.e. 2 minutes, 4 minutes, 6 minutes, 8 minutes, 10minutes, 12minutes and 15minutes under the laminar air flow cabinet,  followed by a dip in 1% KCI for 1 minute. For surface sterilization study, the explants were cultured on MS medium supplemented with 1.0mg/l Kinetin along with 0.5mg/l NAA for initial culture establishment for 30 days and observations were recorded on various sterilization characters.  For shoot proliferation study, the explants were surface sterilized with the best treatment and cultured on MS medium alone or in combination with NAA (0.5 and 1.0mg/ l) and IAA (0.1 and 0.2mg/l) for 30 days. Then, 4.5-6.5 cm shoots were cultured on MS medium alone or in combination with NAA (0.5, 1.0, 1.5, and 2.0mg/l) and IAA (0.25, 0.5, 1.0, and 1.5mg/l) for root formation study for 30 days. The results revealed that 0.1% HgC12 for 6 minutes  was most effective for sterilization of the single node explants of B. galpinii , where the treatment recorded no contamination and death, higher percentage of aseptic culture (100.0%) and survival (100.0%), early bud initiation (11.10 days) , production of more number of shoots / explants (2.07) and longer shoot(5.37 cm) . MS medium fortified with Kinetin 1.0mg/l and IAA 0.1mg/1 (3.30) was the best combination for early shoot initiation(8.67 days), production of maximum number of shoots / explant (2.00), longer shoot (8.00 cm) , more number of branches / shoot (1.97) and leaves per shoot mass(8.10) of light green colour . A branch containing 10 nodes can produce 20 shoots in in vitro condition which is not possible in in vivo condition. Within a period of 30 days, none of the explants developed root primordial, hence further studies on this aspect are essential.

    Keywords

  • Bauhinia galpinii, explants, invitro.

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